근관충전용 시멘트의 치주인대 섬유아세포 독성에 관한 연구
THE CYTOTOXlC EFFECTS OF ROOT CANAL SEALERS ON FlBROBLASTS lN HUMAN PERlODONTAL LlGAMENT
김일현, 김덕교,
소속 상세정보
김일현 ( ) - 경희대학교 식품공학과
김덕교 ( ) - 경희대학교
KMID : 0362319930180010156
Abstract
The purpose of this study was to evaluate for the cytotoxicity of root canal sealers-Tubliseal, N2, AH26, and Sealapex - on fibroblasts cultivated from human periodontal ligament. Succinate dehydrogenase activity test and 51Cr release test were
performed to evaluate the shortterm cytotoxicity. According to 1,3 and 6 days vital cell count and 14C-leucine incorporation rate to fibroblasts were evaluated.
@ES The results of this study were as follows:
@EN 1. In the test of SDH activity by millipore filter method, Sealapex showed mild cytotoxicity but Tubliseal, N2 and AH26 showed severe cytotoxicity.
2. In 51Cr release test, Tubliseal was the most cytotoxic sealer tested, and rank ordered the relative cytotoxicity of the other sealers tested as follows: AH26, N2, Sealapex.
3. In the test of viable cell count, cytotoxicity in Tubliseal was continued because vital cell number reduced with time. Because vital cell was not showed in N2 and AH 26 at 1 day, it was recognized that N2 and AH26 exhibited sever
cytotoxicity.
In
Sealapex, vital cell number increased remarkably with time, so it showed that cytotoxicity decreased with time.
4. In 14C-leucine incorporation rate test, protein sythesis was not produced in Tubliseal, N2 and AH26 after 3 days, it showed that cytotoxicity in Tubliseal, N2 and AH 26 was severe. Although protein synthesis in Sealapex decreased with time,
it
continued after 6 days. Therefore Sealapex has been exhibited mild cytotoxicity.
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